Application of a Simple Oligonucleotide-Directed Mutagenesis Method for Correction of a Frameshift Mutation

Authors

  • S Abu Bakar
  • H Y Chee

Abstract

Application of a rapid and simple method for generating site-directed mutations in double-stranded plasmid DNA is described. Insertion of four nucleotides correcting a frameshift mutation was accomplished utilizing only a single synthetic oligonucleotide. Insertion was confirmed by restriction endonuclease digestion and dideoxy nucleotide sequencing. Successful application of this rapid oIigonucIeotidedirected site-specific mutagenesis requires no subcloning, special bacteria or multiple primers.

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Published

1997-06-30

Issue

Vol. 2 No. 1 (1997)

Section

Research article

License

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